Psychological stress affects the expressions of two kinds of cytokines in rat temporomandibular joint cartilage

doi:10.3969/j.issn.2095-4344.2013.46.005

Abstract

Abstract:

BACKGROUND: Animal modeling has reported that psychological stress can lead temporomandibular joint disease in rats, and there will be pathological changes.
OBJECTIVE: To observe the effects of psychological stress on the expressions of vascular endothelial growth factor and transforming growth factor-β1 in rat temporomandibular joint condylar chondrocytes
METHODS: Forty-eight adult male Wistar rats were randomly divided into the experimental group and the control group, 6 rats in the experimental group and 12 rats in the control group. Thirty-six Wister male adult rats in the experimental group were subjected to the following stimulating factors, such as electroshock, keeping stillness, climate change and food tempting in order to make them under psychological stress. Then, the experimental group rats were executed at 3 weeks after stimulation (3 weeks stimulation group), 6 weeks after stimulation (6 weeks stimulation group) and 6 weeks normal feeding after 6 week stimulation (recovery group), 12 rats were sacrificed at each time point. The 12 rats in the control group were executed at the same time as the last experimental group. The temporomandibular joint specimens were taken to produce slices.
RESULTS AND CONCLUSION: The vascular endothelial growth factor-positive rate of recovery group was significantly larger than that of the 3 weeks stimulation group and the control group (P < 0.05); transforming growth factor-β1 positive rate in the 6 weeks stimulation group was significantly greater than that in the control group (P < 0.05). It indicated that vascular endothelial growth factor was related with the new blood capillaries repairing tissue, and transforming growth factor-β1 might play a role that stimulates bone resorption in psychological stress.

Key words: stress, psychological, temporomandibular joint, vascular endothelial growth factors, transforming growth factor beta1

CLC Number:

R318

Huang Xu, Xiao Peng, Wang Yan, Zhang Hong-yu, Liu Hai-xia. Psychological stress affects the expressions of two kinds of cytokines in rat temporomandibular joint cartilage[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(46): 8004-8011.

Figures/Tables 6

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